rabbit antibody against py416  (Cell Signaling Technology Inc)

Journal: Neuroscience

Article Title: Linkage of non-receptor tyrosine kinase Fyn to mGlu5 receptors in striatal neurons in a depression model

doi: 10.1016/j.neuroscience.2020.02.048

Figure Lengend Snippet: (A) Effects of social isolation on pY416, Fyn, and Src levels in the CPu. (B) Effects of social isolation on pY416, Fyn, and Src levels in the NAc. Note that social isolation (SI) had no significant effect on phosphorylation and expression of Fyn and Src in the CPu (A) and NAc (B) as compared to control (Con) rats. Representative immunoblots are shown left to the quantified data. Data were statistically analyzed using Student’s t-test (n = 6 per group) with ‘n’ equal to the number of animals.

Article Snippet: A rabbit antibody against pY416 (RRID:AB_10013641; Cell Signaling) reacts with the SFK members when phosphorylated at a conserved activation residue, a pan Y416 site.

Techniques: Isolation, Expressing, Western Blot

Journal: Journal of molecular neuroscience : MN

Article Title: Muscarinic acetylcholine receptors inhibit Fyn activity in the rat striatum in vivo

doi: 10.1007/s12031-018-1053-y

Figure Lengend Snippet: (A) Effects of coadministration of SKF81297 and scopolamine on phosphorylation of SFK Y416 and expression of Fyn and Src in the CPu. (B) Effects of coadministration of SKF81297 and scopolamine on phosphorylation of SFK Y416 and expression of Fyn and Src in the NAc. Representative immunoblots are shown to the left of the quantified data. Note that SKF81297 and scopolamine when coadministered induced a marked increase in pY416 levels in both the CPu and NAc, while the two agents injected alone had no effect. Rats were administered with SKF81297 (SKF, 1 mg/kg, i.p.) or scopolamine (Sco, 1 mg/kg, i.p.) or the two agents together and were sacrificed 45 min after drug injection for Western blot analysis. Data are presented as means ± SEM (n = 4 per group). *P < 0.05 versus saline.

Article Snippet: A rabbit polyclonal antibody against pY416 (Cell Signaling) was used.

Techniques: Phospho-proteomics, Expressing, Western Blot, Injection, Saline

Journal: Journal of molecular neuroscience : MN

Article Title: Muscarinic acetylcholine receptors inhibit Fyn activity in the rat striatum in vivo

doi: 10.1007/s12031-018-1053-y

Figure Lengend Snippet: (A) Effects of scopolamine on phosphorylation of SFK Y416 and expression of Fyn and Src in the CPu. (B) Effects of scopolamine on phosphorylation of SFK Y416 and expression of Fyn and Src in the NAc. Representative immunoblots are shown to the left of the quantified data. Note that scopolamine induced a marked increase in pY416 levels in both the CPu and NAc. Rats were administered with scopolamine (5 mg/kg, i.p.) and were sacrificed 45 min after scopolamine injection for Western blot analysis. Data are presented as means ± SEM (n = 4 per group). *P < 0.05 versus saline.

Article Snippet: A rabbit polyclonal antibody against pY416 (Cell Signaling) was used.

Techniques: Phospho-proteomics, Expressing, Western Blot, Injection, Saline

Journal: Journal of molecular neuroscience : MN

Article Title: Muscarinic acetylcholine receptors inhibit Fyn activity in the rat striatum in vivo

doi: 10.1007/s12031-018-1053-y

Figure Lengend Snippet: (A) Effects of atropine on phosphorylation of SFK Y416 and expression of Fyn and Src in the CPu. (B) Effects of atropine on phosphorylation of SFK Y416 and expression of Fyn and Src in the NAc. Representative immunoblots are shown to the left of the quantified data. Note that atropine induced a marked increase in pY416 levels in both the CPu and NAc. Rats were administered with atropine (5 mg/kg, i.p.) and were sacrificed 45 min after scopolamine injection for Western blot analysis. Data are presented as means ± SEM (n = 4 per group). *P < 0.05 versus saline.

Article Snippet: A rabbit polyclonal antibody against pY416 (Cell Signaling) was used.

Techniques: Phospho-proteomics, Expressing, Western Blot, Injection, Saline